UVM Theses and Dissertations
We focus our research on the multiple antibiotic resistance (mar) network of Escherichia coli as a model for transient resistance. The mar network is composed of more than 40 genes, many of which are implicated in resistance to antibiotics. This network is regulated by the three transcription factors, MarA, SoxS, and Rob, but antibiotic resistance is primarily derived from MarA. We experimentally quantify noise in the expression of the mar operon at single cell resolution and show that there is significant variability within the population. We then observe the, difference in noise dynamics in cells with the mar operon present or absent, as well as the contributions to the noise by sox and rob. Finally, we present initial data measuring the propagation of noise in marA to other genes in the mar network (inaA, micF and acrAB) and discuss implications in the generation of different antibiotic resistance phenotypes.